![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K3990004:Design
Designed by: Li Wenxi Group: iGEM21_SMS_Shenzhen (2021-10-01)
LaccaseCotA-386-497
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 885
Illegal AgeI site found at 1192
Illegal AgeI site found at 1348 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We mutated - LEU386>GLU plus HIS497>ASP to improve the enzyme activities.
Source
It comes from Bacillus sp.